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Wednesday, March 6, 2019

Bacteria Growth and Glucose Percentages

Bacterias issue Affected by Various Glucose Percentages This lab tested whether or not different glucose levels activated bacteria egress. My lab assort and I precious to figure out, if more glucose was spread on the bacteria nursing homes, would there be more bacteria growth in the agar habitation. My group predicted that with 25% glucose (the highest division of glucose), the bacteria growth would be the greatest. In order to c entirely for this experiment, my group had 3 ager bases. One plate had 0% glucose, the next plate had 5% glucose and the third plate had 25% glucose in it.Once we received all the plates, we light an alcohol burner. We then used a pipet to uninventive collect bacteria from the culture tubes. We then unresolved the first ager plate (0% glucose) and transferred 10 drops of bacteria culture onto the plate. Then we did the same for the next 2 plates (5% and 25% glucose). Next, we sterilized the spreader by waving it in the flak (alcohol burner) for 15 seconds. Then, we let the spreader cool for 10 seconds, and then opened the first agar plate and spread the bacteria around the plate by quickly and slightly sliding the spreader back and forth across the plate for 5 seconds.Then we closed the plate and did the same subject for the next 2 plates. Next, we sealed each plate with a case of Para film and then placed the plates with bacteria on the boldness of the room until the following week. After waiting a week, we received our groups ager plates back. We noticed that with our experiment, the most sum of money of bacteria growth had no glucose in it. Our results were the following with no glucose, there was 25% bacteria growth, with 5% glucose added to the ager plates, there was only 15% bacteria growth, and with 25% glucose added to the ager plates, only 1% of bacteria growth was present.The to the lowest degree amount of bacteria growth contained the largest percentage of glucose. Based off of our groups results, we can rej ect our hypothesis. We know that our prediction was not what our results showed us. Our prediction was that 25% glucose would have the most amount of bacteria growth, however we can now conclude that bacteria growth does not need any glucose to reproduce. In fact, the more glucose there is, the least amount of bacteria growth occurs. If glucose is present in the bacteria, it is possible for bacteria to reproduce however bacteria will reproduce the greatest when no glucose is present at all.

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